fibulin 5 (Santa Cruz Biotechnology)
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Fibulin 5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibulin+5/Fibulin-5+Antibody/10__15761_slash_cogrm__1000349-68-36-37
Average 93 stars, based on 29 article reviews
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Immunofluorescence:Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide Article Snippet: .. Immunofluorescence labeling of cell layers AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and Labeling:Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide Article Snippet: .. Immunofluorescence labeling of cell layers AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and Cell Culture:Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide Article Snippet: .. Immunofluorescence labeling of cell layers AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide Article Snippet: .. AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and Immunolabeling:Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide Article Snippet: .. Immunofluorescence labeling of cell layers AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide Article Snippet: .. AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and Tissue:Article Title: Expression of Fibulin-3 Fibulin-5 and HOXA11 in vaginal epithelium from women of diverse ethnicity: Implications for racial disparities in pelvic organ prolapse Article Snippet: .. Tissue specimens were air dried for 15 min, hydrated in Tris saline (pH 7.4) for 5 min, and then incubated for 1 hour in a humidified chamber at 37°C with either a goat polyclonal antibody to Saline:Article Title: Expression of Fibulin-3 Fibulin-5 and HOXA11 in vaginal epithelium from women of diverse ethnicity: Implications for racial disparities in pelvic organ prolapse Article Snippet: .. Tissue specimens were air dried for 15 min, hydrated in Tris saline (pH 7.4) for 5 min, and then incubated for 1 hour in a humidified chamber at 37°C with either a goat polyclonal antibody to Incubation:Article Title: Expression of Fibulin-3 Fibulin-5 and HOXA11 in vaginal epithelium from women of diverse ethnicity: Implications for racial disparities in pelvic organ prolapse Article Snippet: .. Tissue specimens were air dried for 15 min, hydrated in Tris saline (pH 7.4) for 5 min, and then incubated for 1 hour in a humidified chamber at 37°C with either a goat polyclonal antibody to Article Title: Fibulin-5 is a tumour suppressor inhibiting cell migration and invasion in ovarian cancer. Article Snippet: To cite: Heo JH, Song J-ye, Jeong J-yeong, et al. J Clin Pathol Published Online First: [please include Day Month Year] doi:10.1136/ jclinpath-2015-203129 ABSTRACT Aims Fibulin-5 is an extracellular matrix (ECM) glycoprotein which has a role in the organisation and stabilisation of ECM structures and regulating cell proliferation and tumourigenesis.. Here, the expression of fibulin-5 and its functional effects on the migration and invasion of ovarian cancer cells were assessed.. Methods Expression of fibulin-5 was detected in 44 ovarian tumour tissues by qRT-PCR, Western blotting and immunohistochemistry. Blocking Assay:Article Title: Fibulin-5 is a tumour suppressor inhibiting cell migration and invasion in ovarian cancer. Article Snippet: To cite: Heo JH, Song J-ye, Jeong J-yeong, et al. J Clin Pathol Published Online First: [please include Day Month Year] doi:10.1136/ jclinpath-2015-203129 ABSTRACT Aims Fibulin-5 is an extracellular matrix (ECM) glycoprotein which has a role in the organisation and stabilisation of ECM structures and regulating cell proliferation and tumourigenesis.. Here, the expression of fibulin-5 and its functional effects on the migration and invasion of ovarian cancer cells were assessed.. Methods Expression of fibulin-5 was detected in 44 ovarian tumour tissues by qRT-PCR, Western blotting and immunohistochemistry. Western Blot:Article Title: Changes in antioxidant, inflammatory and metabolic markers during 1 week cultivation of human skin explants. Article Snippet: Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic Department of Plastic and Aesthetic Surgery, University Hospital Olomouc, I. P. Pavlova 6, 779 00 Olomouc, Czech Republic Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic 4 Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry,, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic Correspondence Alena Rajnochová Svobodová, Department of Medical Chemistry and Biochemistry, Palacký University, Hněvotínská, 3 775 15 Olomouc, Czech Republic.. Email: alf.svoboda@seznam.cz Funding information Faculty of Medicine and Dentistry, Palacký University in Olomouc, Grant/Award Number: IGA_LF_2017_011, IGA_LF_2018_012; Grantová Agentura České Republiky, Grant/ Award Number: GACR 15‐10897S; Universita Palackého v Olomouci, Grant/Award Number: RVO 61989592; Institutional Support of Palacký University in Olomouc, Grant/Award Number: RVO 61989592; Ministry of Education, Youth and Sports of the Czech Republic, Grant/Award Number: LO1304 |
