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fibulin 5  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology fibulin 5
    Fibulin 5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fibulin+5/Fibulin-5+Antibody/10__15761_slash_cogrm__1000349-68-36-37
    Average 93 stars, based on 29 article reviews
    fibulin 5 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide
    Article Snippet: .. Immunofluorescence labeling of cell layers AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and fibulin-5 (1:100 v/v dilution, Santa Cruz Biotechnology). .. Cell layers were washed with PBS and labeled with Alexafluor 633 goat anti-rabbit secondary antibody; 1:1000 dilution, ThermoFisher Scientific).

    Labeling:

    Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide
    Article Snippet: .. Immunofluorescence labeling of cell layers AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and fibulin-5 (1:100 v/v dilution, Santa Cruz Biotechnology). .. Cell layers were washed with PBS and labeled with Alexafluor 633 goat anti-rabbit secondary antibody; 1:1000 dilution, ThermoFisher Scientific).

    Cell Culture:

    Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide
    Article Snippet: .. Immunofluorescence labeling of cell layers AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and fibulin-5 (1:100 v/v dilution, Santa Cruz Biotechnology). .. Cell layers were washed with PBS and labeled with Alexafluor 633 goat anti-rabbit secondary antibody; 1:1000 dilution, ThermoFisher Scientific).

    Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide
    Article Snippet: .. AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and fibulin-5 (1:100 v/v dilution, Santa Cruz Biotechnology). .. Cell layers were washed with PBS and labeled with Alexafluor 633 goat anti-rabbit secondary antibody; 1:1000 dilution, ThermoFisher Scientific).

    Immunolabeling:

    Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide
    Article Snippet: .. Immunofluorescence labeling of cell layers AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and fibulin-5 (1:100 v/v dilution, Santa Cruz Biotechnology). .. Cell layers were washed with PBS and labeled with Alexafluor 633 goat anti-rabbit secondary antibody; 1:1000 dilution, ThermoFisher Scientific).

    Article Title: Alterations in phenotype and gene expression of adult human aneurysmal smooth muscle cells by exogenous nitric oxide
    Article Snippet: .. AAA-SMCs were cultured on glass chamber slides (10 4 cells/well) for 21 days in the presence or absence of 100 nM GSNO, and the cell layers were fixed in cold methanol, washed with PBS, blocked with PBS containing 5% v/v goat serum (ThermoFisher Scientific) and immunolabeled with rabbit anti-human polyclonal antibodies against elastin (1:100 v/v dilution, Abcam), fibrillin-1 (1:100 v/v dilution, Bioss Antibodies), fibulin-4 (1:100 v/v dilution, Santa Cruz Biotechnology), and fibulin-5 (1:100 v/v dilution, Santa Cruz Biotechnology). .. Cell layers were washed with PBS and labeled with Alexafluor 633 goat anti-rabbit secondary antibody; 1:1000 dilution, ThermoFisher Scientific).

    Tissue:

    Article Title: Expression of Fibulin-3 Fibulin-5 and HOXA11 in vaginal epithelium from women of diverse ethnicity: Implications for racial disparities in pelvic organ prolapse
    Article Snippet: .. Tissue specimens were air dried for 15 min, hydrated in Tris saline (pH 7.4) for 5 min, and then incubated for 1 hour in a humidified chamber at 37°C with either a goat polyclonal antibody to Fibulin-5 (Santa Cruz, CA), a mouse monoclonal antibody to fibulin-3 (Santa Cruz, CA) or a rabbit polyclonal antibody to HOXA11(Abcam, MA). ..

    Saline:

    Article Title: Expression of Fibulin-3 Fibulin-5 and HOXA11 in vaginal epithelium from women of diverse ethnicity: Implications for racial disparities in pelvic organ prolapse
    Article Snippet: .. Tissue specimens were air dried for 15 min, hydrated in Tris saline (pH 7.4) for 5 min, and then incubated for 1 hour in a humidified chamber at 37°C with either a goat polyclonal antibody to Fibulin-5 (Santa Cruz, CA), a mouse monoclonal antibody to fibulin-3 (Santa Cruz, CA) or a rabbit polyclonal antibody to HOXA11(Abcam, MA). ..

    Incubation:

    Article Title: Expression of Fibulin-3 Fibulin-5 and HOXA11 in vaginal epithelium from women of diverse ethnicity: Implications for racial disparities in pelvic organ prolapse
    Article Snippet: .. Tissue specimens were air dried for 15 min, hydrated in Tris saline (pH 7.4) for 5 min, and then incubated for 1 hour in a humidified chamber at 37°C with either a goat polyclonal antibody to Fibulin-5 (Santa Cruz, CA), a mouse monoclonal antibody to fibulin-3 (Santa Cruz, CA) or a rabbit polyclonal antibody to HOXA11(Abcam, MA). ..

    Article Title: Fibulin-5 is a tumour suppressor inhibiting cell migration and invasion in ovarian cancer.
    Article Snippet: To cite: Heo JH, Song J-ye, Jeong J-yeong, et al. J Clin Pathol Published Online First: [please include Day Month Year] doi:10.1136/ jclinpath-2015-203129 ABSTRACT Aims Fibulin-5 is an extracellular matrix (ECM) glycoprotein which has a role in the organisation and stabilisation of ECM structures and regulating cell proliferation and tumourigenesis.. Here, the expression of fibulin-5 and its functional effects on the migration and invasion of ovarian cancer cells were assessed.. Methods Expression of fibulin-5 was detected in 44 ovarian tumour tissues by qRT-PCR, Western blotting and immunohistochemistry.

    Blocking Assay:

    Article Title: Fibulin-5 is a tumour suppressor inhibiting cell migration and invasion in ovarian cancer.
    Article Snippet: To cite: Heo JH, Song J-ye, Jeong J-yeong, et al. J Clin Pathol Published Online First: [please include Day Month Year] doi:10.1136/ jclinpath-2015-203129 ABSTRACT Aims Fibulin-5 is an extracellular matrix (ECM) glycoprotein which has a role in the organisation and stabilisation of ECM structures and regulating cell proliferation and tumourigenesis.. Here, the expression of fibulin-5 and its functional effects on the migration and invasion of ovarian cancer cells were assessed.. Methods Expression of fibulin-5 was detected in 44 ovarian tumour tissues by qRT-PCR, Western blotting and immunohistochemistry.

    Western Blot:

    Article Title: Changes in antioxidant, inflammatory and metabolic markers during 1 week cultivation of human skin explants.
    Article Snippet: Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic Department of Plastic and Aesthetic Surgery, University Hospital Olomouc, I. P. Pavlova 6, 779 00 Olomouc, Czech Republic Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic 4 Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry,, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic Correspondence Alena Rajnochová Svobodová, Department of Medical Chemistry and Biochemistry, Palacký University, Hněvotínská, 3 775 15 Olomouc, Czech Republic.. Email: alf.svoboda@seznam.cz Funding information Faculty of Medicine and Dentistry, Palacký University in Olomouc, Grant/Award Number: IGA_LF_2017_011, IGA_LF_2018_012; Grantová Agentura České Republiky, Grant/ Award Number: GACR 15‐10897S; Universita Palackého v Olomouci, Grant/Award Number: RVO 61989592; Institutional Support of Palacký University in Olomouc, Grant/Award Number: RVO 61989592; Ministry of Education, Youth and Sports of the Czech Republic, Grant/Award Number: LO1304



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    Impact <t>of</t> <t>fibulin‐5</t> deficiency on the skin aging process. (A) Schematic representation of the interfollicular epidermis of mouse tail skin. Slow‐cycling epidermal stem cells (SCs) produce the K10 + interscale lineage (orange), and fast‐cycling epidermal SCs produce the K36 + scale lineage (blue). (B, C) Immunostaining of fibulin‐5 (green) in sections of mouse tail skin from 2‐month‐old versus 30‐month‐old C57BL/6J mice and quantification (C). The white dashed line represents the epidermal–dermal boundary. Scale bars: 50 μm. (D, E) Immunostaining of fibulin‐5 (green) in sections of mouse tail skin from 3‐month‐old Fbln5 WT versus KO mice and quantification (E). The white dashed line represents the epidermal–dermal boundary and hair follicles. Scale bars: 50 μm. (F) Images of 12‐month‐old Fbln5 WT and KO mice. (G) The body weights of 12‐month‐old Fbln5 WT and KO mice. (H–K) Hematoxylin and eosin staining of sagittal sections of the skin of 3‐ and 12‐month‐old Fbln5 WT versus KO mice and quantification (I, K). Scale bars: 150 μm. Epidermal thickness was measured in interscale and scale regions. (L–O) Whole‐mount staining of BrdU (green, a proliferation marker) and Hoechst (blue) in 3‐ and 12‐month‐old Fbln5 WT versus KO mice and quantification (M, O). Scale bars: 200 μm. (P–U) Whole‐mount staining of K10 (green, interscale lineage), K36 (red, scale lineage), and Hoechst (blue) in 2‐month‐old versus 30‐month‐old C57BL/6J mice and 3‐ and 12‐month‐old Fbln5 WT versus KO mice and quantification (Q, S, U). Scale bars: 200 μm. All data are presented as the mean ± SD. Each dot represents one mouse. Statistical significance is assessed using a two‐tailed unpaired t ‐test (C, E, G, I, K, M, O, Q, S, U). *, p < 0.05; **, p < 0.01; ns, not significant.
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    Image Search Results


    Impact of fibulin‐5 deficiency on the skin aging process. (A) Schematic representation of the interfollicular epidermis of mouse tail skin. Slow‐cycling epidermal stem cells (SCs) produce the K10 + interscale lineage (orange), and fast‐cycling epidermal SCs produce the K36 + scale lineage (blue). (B, C) Immunostaining of fibulin‐5 (green) in sections of mouse tail skin from 2‐month‐old versus 30‐month‐old C57BL/6J mice and quantification (C). The white dashed line represents the epidermal–dermal boundary. Scale bars: 50 μm. (D, E) Immunostaining of fibulin‐5 (green) in sections of mouse tail skin from 3‐month‐old Fbln5 WT versus KO mice and quantification (E). The white dashed line represents the epidermal–dermal boundary and hair follicles. Scale bars: 50 μm. (F) Images of 12‐month‐old Fbln5 WT and KO mice. (G) The body weights of 12‐month‐old Fbln5 WT and KO mice. (H–K) Hematoxylin and eosin staining of sagittal sections of the skin of 3‐ and 12‐month‐old Fbln5 WT versus KO mice and quantification (I, K). Scale bars: 150 μm. Epidermal thickness was measured in interscale and scale regions. (L–O) Whole‐mount staining of BrdU (green, a proliferation marker) and Hoechst (blue) in 3‐ and 12‐month‐old Fbln5 WT versus KO mice and quantification (M, O). Scale bars: 200 μm. (P–U) Whole‐mount staining of K10 (green, interscale lineage), K36 (red, scale lineage), and Hoechst (blue) in 2‐month‐old versus 30‐month‐old C57BL/6J mice and 3‐ and 12‐month‐old Fbln5 WT versus KO mice and quantification (Q, S, U). Scale bars: 200 μm. All data are presented as the mean ± SD. Each dot represents one mouse. Statistical significance is assessed using a two‐tailed unpaired t ‐test (C, E, G, I, K, M, O, Q, S, U). *, p < 0.05; **, p < 0.01; ns, not significant.

    Journal: Aging Cell

    Article Title: Integrin‐Binding Matricellular Protein Fibulin‐5 Maintains Epidermal Stem Cell Heterogeneity During Skin Aging

    doi: 10.1111/acel.70483

    Figure Lengend Snippet: Impact of fibulin‐5 deficiency on the skin aging process. (A) Schematic representation of the interfollicular epidermis of mouse tail skin. Slow‐cycling epidermal stem cells (SCs) produce the K10 + interscale lineage (orange), and fast‐cycling epidermal SCs produce the K36 + scale lineage (blue). (B, C) Immunostaining of fibulin‐5 (green) in sections of mouse tail skin from 2‐month‐old versus 30‐month‐old C57BL/6J mice and quantification (C). The white dashed line represents the epidermal–dermal boundary. Scale bars: 50 μm. (D, E) Immunostaining of fibulin‐5 (green) in sections of mouse tail skin from 3‐month‐old Fbln5 WT versus KO mice and quantification (E). The white dashed line represents the epidermal–dermal boundary and hair follicles. Scale bars: 50 μm. (F) Images of 12‐month‐old Fbln5 WT and KO mice. (G) The body weights of 12‐month‐old Fbln5 WT and KO mice. (H–K) Hematoxylin and eosin staining of sagittal sections of the skin of 3‐ and 12‐month‐old Fbln5 WT versus KO mice and quantification (I, K). Scale bars: 150 μm. Epidermal thickness was measured in interscale and scale regions. (L–O) Whole‐mount staining of BrdU (green, a proliferation marker) and Hoechst (blue) in 3‐ and 12‐month‐old Fbln5 WT versus KO mice and quantification (M, O). Scale bars: 200 μm. (P–U) Whole‐mount staining of K10 (green, interscale lineage), K36 (red, scale lineage), and Hoechst (blue) in 2‐month‐old versus 30‐month‐old C57BL/6J mice and 3‐ and 12‐month‐old Fbln5 WT versus KO mice and quantification (Q, S, U). Scale bars: 200 μm. All data are presented as the mean ± SD. Each dot represents one mouse. Statistical significance is assessed using a two‐tailed unpaired t ‐test (C, E, G, I, K, M, O, Q, S, U). *, p < 0.05; **, p < 0.01; ns, not significant.

    Article Snippet: For the fibulin‐5 coating assay, the 12‐well plates were coated overnight at 4°C with collagen type IV (50 μg/mL in PBS) either alone or with 90 ng/mL recombinant human fibulin‐5 (R&D Systems) in PBS.

    Techniques: Immunostaining, Staining, Marker, Two Tailed Test

    Changes in integrin and extracellular matrix expression due to the loss of fibulin‐5. (A) The heatmap shows changes in integrins and ECM proteins in 12‐month‐old Fbln5 WT and KO epidermal stem cells. Genes with a ≥ 2‐fold change are used for analysis. (B) Schematic representation of the epidermal–dermal junction and its associated proteins. (C–V) Immunostaining and quantification of the indicated proteins: Collagen XVII (C–F; green), integrin β1 (G–J; green), integrin α6 (K–N; red) integrin β3 (O–R; green), nectin‐3 (S–V; green), K5 (S–V; gray), and K36 (S–V; red, scale lineage). The white dashed lines represent the epidermal–dermal boundary. Scale bars: 50 μm. All data are presented as the mean ± SD. Each dot represents one mouse. Statistical significance is assessed using a two‐tailed unpaired t ‐test (D, F, H, J, N, P, R, T, V) or Mann–Whitney U test (L). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. The schematic in panel B is created with BioRender.com .

    Journal: Aging Cell

    Article Title: Integrin‐Binding Matricellular Protein Fibulin‐5 Maintains Epidermal Stem Cell Heterogeneity During Skin Aging

    doi: 10.1111/acel.70483

    Figure Lengend Snippet: Changes in integrin and extracellular matrix expression due to the loss of fibulin‐5. (A) The heatmap shows changes in integrins and ECM proteins in 12‐month‐old Fbln5 WT and KO epidermal stem cells. Genes with a ≥ 2‐fold change are used for analysis. (B) Schematic representation of the epidermal–dermal junction and its associated proteins. (C–V) Immunostaining and quantification of the indicated proteins: Collagen XVII (C–F; green), integrin β1 (G–J; green), integrin α6 (K–N; red) integrin β3 (O–R; green), nectin‐3 (S–V; green), K5 (S–V; gray), and K36 (S–V; red, scale lineage). The white dashed lines represent the epidermal–dermal boundary. Scale bars: 50 μm. All data are presented as the mean ± SD. Each dot represents one mouse. Statistical significance is assessed using a two‐tailed unpaired t ‐test (D, F, H, J, N, P, R, T, V) or Mann–Whitney U test (L). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. The schematic in panel B is created with BioRender.com .

    Article Snippet: For the fibulin‐5 coating assay, the 12‐well plates were coated overnight at 4°C with collagen type IV (50 μg/mL in PBS) either alone or with 90 ng/mL recombinant human fibulin‐5 (R&D Systems) in PBS.

    Techniques: Expressing, Immunostaining, Two Tailed Test, MANN-WHITNEY

    Extracellular fibulin‐5 enhances YAP activity and fast‐cycling stem cell‐associated gene expression in human keratinocytes. (A–H) Immunostaining of YAP (A, green), SLC1A3 (C, red), Ki‐67 (E, gray), and ASS1 (G, green) in human keratinocytes and quantification (B, D, F, H). Cells are seeded at 150,000, 50,000, and 25,000 cells per well in 12‐well plates and cultured for 48 h before analysis. Scale bars: 50 μm. (I, J) Immunostaining of YAP in primary human keratinocytes and quantification (J). Cells are seeded at 50,000 cells per well in 12‐well plates and cultured for 24 h and then treated with verteporfin or vehicle control for 8 h. Nuclear YAP (%) was calculated as the proportion of cells with nuclear YAP localization among all Hoechst + nuclei. Scale bars: 50 μm. (K–M) RT‐qPCR analysis of CTGF , SLC1A3 , and ASS1 following 8 h of verteporfin treatment. (N, O) Immunostaining of YAP in primary human keratinocytes and quantification (O). Cells are seeded at 300,000 cells per well on collagen IV–coated plates with or without recombinant human fibulin‐5 and cultured to ~80% confluence. The medium is then replaced, and cells are analyzed 8 h later. Scale bars: 50 μm. (P–R) RT‐qPCR analysis of CTGF , SLC1A3 , and ASS1 following culture on plates coated with collagen IV ± fibulin‐5. All data are presented as the mean ± SD. Each dot represents one independent biological replicate. Statistical significance is assessed using a two‐tailed unpaired t ‐test (K, L, P, Q, R), Welch's t ‐test (J, M, O), or one‐way ANOVA (B, D, F, H). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant.

    Journal: Aging Cell

    Article Title: Integrin‐Binding Matricellular Protein Fibulin‐5 Maintains Epidermal Stem Cell Heterogeneity During Skin Aging

    doi: 10.1111/acel.70483

    Figure Lengend Snippet: Extracellular fibulin‐5 enhances YAP activity and fast‐cycling stem cell‐associated gene expression in human keratinocytes. (A–H) Immunostaining of YAP (A, green), SLC1A3 (C, red), Ki‐67 (E, gray), and ASS1 (G, green) in human keratinocytes and quantification (B, D, F, H). Cells are seeded at 150,000, 50,000, and 25,000 cells per well in 12‐well plates and cultured for 48 h before analysis. Scale bars: 50 μm. (I, J) Immunostaining of YAP in primary human keratinocytes and quantification (J). Cells are seeded at 50,000 cells per well in 12‐well plates and cultured for 24 h and then treated with verteporfin or vehicle control for 8 h. Nuclear YAP (%) was calculated as the proportion of cells with nuclear YAP localization among all Hoechst + nuclei. Scale bars: 50 μm. (K–M) RT‐qPCR analysis of CTGF , SLC1A3 , and ASS1 following 8 h of verteporfin treatment. (N, O) Immunostaining of YAP in primary human keratinocytes and quantification (O). Cells are seeded at 300,000 cells per well on collagen IV–coated plates with or without recombinant human fibulin‐5 and cultured to ~80% confluence. The medium is then replaced, and cells are analyzed 8 h later. Scale bars: 50 μm. (P–R) RT‐qPCR analysis of CTGF , SLC1A3 , and ASS1 following culture on plates coated with collagen IV ± fibulin‐5. All data are presented as the mean ± SD. Each dot represents one independent biological replicate. Statistical significance is assessed using a two‐tailed unpaired t ‐test (K, L, P, Q, R), Welch's t ‐test (J, M, O), or one‐way ANOVA (B, D, F, H). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, not significant.

    Article Snippet: For the fibulin‐5 coating assay, the 12‐well plates were coated overnight at 4°C with collagen type IV (50 μg/mL in PBS) either alone or with 90 ng/mL recombinant human fibulin‐5 (R&D Systems) in PBS.

    Techniques: Activity Assay, Gene Expression, Immunostaining, Cell Culture, Control, Quantitative RT-PCR, Recombinant, Two Tailed Test

    Proposed model of cellular and molecular alterations associated with fibulin‐5 deficiency during skin aging. In young skin, slow‐cycling and fast‐cycling epidermal stem cells (SCs) are spatially compartmentalized and give rise to their respective lineages. During aging, decreased fibulin‐5 expression is associated with altered integrin and extracellular matrix (ECM) protein expression, potentially affecting intracellular signaling through fibulin‐5–integrin interactions. Reduced YAP activity is associated with a decrease in the fast‐cycling epidermal stem cell compartment in aged skin and human keratinocytes. The schematic is created with BioRender.com .

    Journal: Aging Cell

    Article Title: Integrin‐Binding Matricellular Protein Fibulin‐5 Maintains Epidermal Stem Cell Heterogeneity During Skin Aging

    doi: 10.1111/acel.70483

    Figure Lengend Snippet: Proposed model of cellular and molecular alterations associated with fibulin‐5 deficiency during skin aging. In young skin, slow‐cycling and fast‐cycling epidermal stem cells (SCs) are spatially compartmentalized and give rise to their respective lineages. During aging, decreased fibulin‐5 expression is associated with altered integrin and extracellular matrix (ECM) protein expression, potentially affecting intracellular signaling through fibulin‐5–integrin interactions. Reduced YAP activity is associated with a decrease in the fast‐cycling epidermal stem cell compartment in aged skin and human keratinocytes. The schematic is created with BioRender.com .

    Article Snippet: For the fibulin‐5 coating assay, the 12‐well plates were coated overnight at 4°C with collagen type IV (50 μg/mL in PBS) either alone or with 90 ng/mL recombinant human fibulin‐5 (R&D Systems) in PBS.

    Techniques: Expressing, Activity Assay